Effects of Urtica dioica Seeds on Oxidative/ Nitrosative Stress Levels and Myeloperoxidase Activity in Muscle Ischemia/Reperfusion Injury

The purpose of this study is to evaluate the preventive effects Urtica dioica (UD) on muscle ischemia/reperfusion (I/R) injury. A total 27 male Wistar rats were divided into three groups as control group (1), I/R + saline (2), and I/R+UD (3). Group 1 did not receive any treatment. 2 was administered a 2mL/kg (1mL/kg before ischemia mL/kg after reperfusion), 3 given 2mL UD 1mL/kg reperfusion) Saline via intraesophageal canula once day for five days. At end days, all exposed 60 min followed by reperfusion bilateral hindlimbs induced using tourniquet. Muscle tissue histopathologies evaluated light microscopy. Furthermore, oxidative/nitrosative stress biomarkers such catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA), nitrotyrosine (3-NT), nitric oxide (NO), myeloperoxidase (MPO) an inflammatory marker in samples measured. treatment significantly decreased biomarker levels MPO (p<0.05). We established that could alleviate injury inhibiting inflammation stress.